Lipase is a digestive enzyme that hydrolyses the ester bonds of triglycerides to release fatty acids and glycerol (propylene glycol).
As the reaction proceeds, the pH decreases as a result of the rising concentration of carboxylic acids.
This means it is very convenient to monitor the progress of the reaction by measuring pH.
To get a visual result, universal indicator or hydrogen carbonate indicator can be used to show a range of colours caused by the pH changing as the reaction proceeds.
With this enzyme, we suggest using full cream milk as the source of triglycerides.
- This particular type of lipase is sensitive to pH.
- If the reaction mixture is made too alkaline (pH greater than 10), the enzyme will be denatured and rendered ineffective.
- For this reason, it is very important to ensure that only the required amount of Na2CO3 is used.
- Adding any more than is necessary can cause problems. We recommend using 0.01M sodium carbonate, and depending on your lipids and the volume used, you may only need a few drops to raise the pH high enough but not too high, or you might need a couple of mL.
Qty: 20g
Notes:
- This product is sourced from Rhizopus oryzae
- Refrigerate upon arrival
- When working out the concentration required, start from the basis of our enzymes being "100%"